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KMID : 0380020220370010025
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Korean Journal of Biotechnology and Bioengineering
2022 Volume.37 No. 1 p.25 ~ p.31
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Immunity-enhancing Effect of Hot Water Extract of Barley Sprout
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Seo Ji-Won
Lee Hyo-Jae
Lee Chang-Yeol
Kim Sang-Hyeon
Kim Jong-Tae
Kim Min-Jeong
Jang Seung-Hee
Kim Young-Min
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Abstract
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Barley Sprout is a young sprout of barley and contains various nutrients with antioxidant effects. It also has various physiological effects including antioxidant, anti-obesity, and anti-diabetic effects. This study investigated the immunity-enhancing effect of Hot Water extract of Barley Sprout (BSE). According to the MTT assay results, BSE was not cytotoxic to RAW 264.7 macrophage cell line. Therefore, subsequent experiments, including the NO (nitric oxide) assay, Western blot assay, and WST-1 assay, were then conducted. First, to confirm how BSE affects the production of NO and Cytokine expression, which are biomarkers of immune cell activity, we treated the cells with LPS (lipopolysaccharide), which served as the positive control, and BSE at varying concentrations. The results show that BSE increased the production of NO and Cytokines, such as TNF-α (Tumor necrosis factor-alpha), IL-6 (Interleukin-6) and IL-1β (Interleukin-1beta). Additionally, the proliferation of Molt-4 cell line (T-cell) and Raji cell line (B-cell) increased in a concentration-dependent manner compared with the control. Taken together, these findings suggest that BSE consumption can improve immunity by enhancing innate immunity via activation of macrophages.
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KEYWORD
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Barley Sprout, cytokine, immunity-enhancing, nitric oxide, RAW 264.7 mouse macrophage cell
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